Alcaligenes eutrophus as a Bacterial Chromate Sensor
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Alcaligenes eutrophus as a bacterial chromate sensor.
In Alcaligenes eutrophus CH34, determinants encoding inducible resistance to chromate (chr) and to cobalt and nickel (cnr) are located adjacent to each other on plasmid pMOL28. To develop metal-sensing bacterial strains, a cloned part of plasmid pMOL28, which contains both determinants, was mutated with Tn5-lacZ. The chr::lacZ fusions were specifically induced by chromium; cnr was induced best ...
متن کاملDenitrification by Alcaligenes eutrophus is plasmid dependent.
Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of converting some nitrate to nitrite, but they did not metabolize nitrite under anaerobic...
متن کاملDissimilation of aromatic compounds by Alcaligenes eutrophus.
The range of aromatic compounds that support the growth of Alcaligenes eutrophus has been determined, and the pathways used for the dissimilation of these substrates have been explored, largely by enzymatic analyses. The beta-ketoadipate pathway operates in the dissimilation of benzoate and p-hydroxybenzoate; the genetisate pathway, in the dissimilation of m-hydroxybenzoate; and the meta cleava...
متن کاملThe Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation.
Nucleotide sequence analysis revealed a 1,791-bp open reading frame in the hox gene cluster of the gram-negative chemolithotroph Alcaligenes eutrophus H16. In order to investigate the biological role of this open reading frame, we generated an in-frame deletion allele via a gene replacement strategy. The resulting mutant grew significantly more slowly than the wild type under lithoautotrophic c...
متن کاملRibulose-bisphosphate carboxylase manipulation in the hydrogen bacterium Alcaligenes eutrophus.
separated by an intervening stretch of DNA, variable in length depending on the particular species of origin (Curtis & Hazelkorn, 1983; Shinozaki & Sugiura, 1983). The gene from S,.rzc~c.lioc.oc~r~us PCC30 1 (Reichelt & Delaney, 1983) has been cloned into the ampr region of PLa2311 (Remaut ct al., 1981) with expression controlled by the strong temperature-sensitive leftward promoter (PI ) of ba...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.2.453-458.1998